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Invitrogen™ FITC Recombinant Mouse Monoclonal Antibody (NAWESLEE)

Catalog No. 740178M
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74-017-8M 100 μL
74-017-8MXS 20 μL
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Catalog No. 74-017-8M Supplier Invitrogen™ Supplier No. 740178M
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Description

Mouse Recombinant Monoclonal Antibody

Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
TRUSTED_SUSTAINABILITY

Specifications

Antigen FITC
Applications Flow Cytometry, Western Blot, Immunocytochemistry
Classification Recombinant Monoclonal
Clone NAWESLEE
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.09% sodium azide; pH 7.2-7.4
Gene Alias Fluorescein
Host Species Mouse
Purification Method Protein A/G
Quantity 100 μL
Regulatory Status RUO
Primary or Secondary Primary
Target Species Chemical
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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