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Invitrogen™ Fixation Medium (Medium A)
Description
Fixation Medium (Medium A) is a component of the FIX & PERM™ Cell Permeabilization Kit. The kit is used in flow cytometry for fixing and permeabilizing cells in suspension, allowing access of antibody to intracellular targets without sacrificing scatter characteristics.
The FIX & PERM™ Cell Permeabilization Kit is:
Flexible—compatible with multiple sample types and cellular antigens
Robust—scatter pattern of cells unaffected
Efficient—simultaneous detection of intracellular and surface markers
View a selection guide for all available FIX & PERM™ products and products related to sample preparation (including cell lysis and preservation solutions, blocking reagents, and information about the use of Dynabeads™ cell separation products for cell isolation).
The Fixation Medium formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.
How It Works
Cells are incubated for 15 minutes at room temperature with the Fixation Medium and washed. Cells can then be incubated with Permeabilization Medium (Medium B) (Cat. No. GAS002S100) and fluorochrome-labeled antibody. Both Fixation Medium and Permeabilization Medium are included in the FIX & PERM™ Cell Permeabilization Kit.
The FIX & PERM™ Cell Permeabilization Kit is:
View a selection guide for all available FIX & PERM™ products and products related to sample preparation (including cell lysis and preservation solutions, blocking reagents, and information about the use of Dynabeads™ cell separation products for cell isolation).
The Fixation Medium formula was specifically designed to reduce background staining and allow simultaneous addition of fluorochrome-labeled antibodies during the permeabilization step.
How It Works
Cells are incubated for 15 minutes at room temperature with the Fixation Medium and washed. Cells can then be incubated with Permeabilization Medium (Medium B) (Cat. No. GAS002S100) and fluorochrome-labeled antibody. Both Fixation Medium and Permeabilization Medium are included in the FIX & PERM™ Cell Permeabilization Kit.
Specifications
Specifications
| Content And Storage | Store at room temperature. |
| Quantity | 100 mL |
| Volume (Metric) | 100 mL |
| Storage Buffer | Formaldehyde |
| Color | Gray |
| Form | Liquid |
| Reagent Type | Fixation Reagent |
| Shipping Condition | Room Temperature |
| For Use With (Application) | Flow Cytometry |
| Product Line | FIX and PERM |
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Safety and Handling
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
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