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  6. Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer 

Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer

Catalog No. 501129748
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50-112-9748 600 mL
50-112-8916 200 mL
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Contains Sodium azide
Catalog No. 50-112-9748 Supplier Invitrogen™ Supplier No. 00422226
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Contains Sodium azide

Description

A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. Designed for use in immunofluorescent staining protocols of cells in suspension.

  • Designed for use in immunofluorescent staining protocols of cells in suspension
  • A buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative
  • This buffer can be used for antibody and cell dilution steps, as well as all the wash steps required for the surface staining and flow cytometric analysis
  • Contains animal serum proteins to help minimizing non-specific binding of antibodies
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Specifications

Quantity 600 mL
Product Line eBioscience
Product Type Staining Buffer

Frequently Asked Questions (FAQs)

Is it necessary to wash OneComp eBeads after staining?

One wash with 2 mL of Flow Cytometry Staining Buffer (Cat. No. 00-4222-26, 00-4222-57) is recommended for ideal staining of OneComp eBeads. However, it may be possible in some cases to use unwashed OneComp eBeads, if the antibody staining concentration is low enough. In testing, it appears that concentrations starting below 0.06 µg per test may provide appropriate compensation values. Usage in this way should be confirmed in the individual antibody of interest.
Can eFluor Organic fluorochromes and eVolve QDots be used in combination with other organic fluorochromes?

Yes, both the eFluor Organic Dyes and eVolve QDots are fully compatible with conventional fluorochromes and can be used in the same manner as the fluorochromes that they are replacing. We recommend the Flow Cytometry Staining Buffer (Cat. No. 00-4222-26, 00-4222-57) as stated in the Staining Cell Surface Antigens for Flow Cytometry Protocol.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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