MilliporeSigma™ Fluorescent Mouse ES/iPS Cell Characterization Kit

Description

Includes

• Fast Red Violet solution: One 15mL bottle
• Napthol AS-BI phosphate solution. One 15mL bottle
• Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor™ 488 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
• Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
• Mouse anti-DPPA-2, clone 6C1.2, Alexa Fluor™ 488 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
• Mouse anti-SSEA-1, clone MC-480, Cy3 conjugate. One vial containing 50μL of 0.5mg/mL conjugated monoclonal antibody
• DAPI, 100μL. One vial containing 100μL volume

Contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse Embryonic stem and induced pluripotent Stem cells

• Contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of mouse ES/iPS cells
• Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and DPPA-2 and cell surface epitope SSEA-1 to enable rapid immunocytochemical marker analysis
• The Dapi nuclear dye is conveniently included to aid in cell quantification
• While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics

Specifications

• Content And Storage: Store at 2° to 8°C until expiration date of kit
• For Use With (Application): Immunocytochemistry
• Quantity: 1 Kit