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R&D Systems™ Formamidopyrimidine DNA Glycosylase FLARE™ Assay Kit

Catalog No. 4040100FK
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75 Tests
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40-401-00FK 75 Tests
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Description

Reagent Kit for Analysis of DNA Damage in Single Cells Using the Trevigen CometAssay™ and E. coli Fpg protein

  • Trevigen’s Fpg FLARE™(Fragment Length Analysis using Repair Enzymes) Assay Kit provides the ability to detect DNA damage in single cells using E. coli formamidopyrimidine-DNA glycosylase (Fpg) in conjunction with Trevigen’s CometAssay™ single cell gel electrophoresis kit
  • To assess the type of DNA damage induced by a putative mutagen, drug, or treatment regimen, cells are harvested after treatment and immobilized in low melting point agarose (LMAgarose) on FLARE™ Slides
  • The cells are gently lysed and then incubated with Fpg to assess the type of damage present.
  • Fpg catalyzes the excision of the following forms of DNA damage:
    • Open ring forms of 7-methylguanine, including 2,6-diamino-4-hydroxy-5- N-methylformamido-pyrimidine, and 4,6-diamino-5-formamidopyimidine, a lethal lesion.
    • 8-oxoguanine, a highly mutagenic lesion and probably the most important biological substrate of Fpg.
    • 5-hydroxycytosine and 5-hydroxyuracil.
    • Aflatoxin-bound imidazole-ring-opened guanine. 
    • Imidazole ring opened N-2-aminofluorene-C8-guanine.

Specifications

For Use With (Equipment) CometAssay Single Cell Gel Electrophoresis Kit
Quantity 75 Tests
Storage Requirements Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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