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Novus Biologicals™ FoxP3
SDP

Catalog No. NBP229545 Shop All Novus Biologicals Products
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Quantity:
2 μg

Description

Foxp3, human (full length cDNA) in PCLXSN Retroviral expression vector

  • RetroMax system takes advantages of two properties of 293 cells (high level of transfectibility and strong E1A-mediated stimulation of CMV promoter controlled transcription)
  • 293 cells of nonmurine origin avoid problem of selective packaging and transfer of VL30 genomes (present in all murine packaging cells)
  • Vector supernatants are free of helper virus and are of sufficiently high titer within 2 days of transient transfection in 293 cells
  • For amplification, transform DH-5alpha or XL-1 Blue and plate on LB plates containing 50mg/mL ampicillin
  • Buffer: 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

Recommended Storage

Store at -20°C. Avoid freeze-thaw cycles.

Specifications

Storage Buffer 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)
Content And Storage Store at -20°C. Avoid freeze-thaw cycles.
Quantity 2 μg
Vector FoxP3 Vector
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