Invitrogen™ FOXP3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1806

Catalog No. PIPA1806

Description

PA1-806 detects FOXP3 from human samples. PA1-806 has been successfully used in Western blot and ChIP procedures. By Western blot, PA1-806 detects a ~50 kDa band representing FOXP3 from Hela cells. The PA1-806 immunogen is a synthetic peptide corresponding to residues W(406) T V D E L E F R K K R S Q R(420) of human FOXP3. PA1-806 can be used with blocking peptide PEP-297.

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

Specifications

• Antigen: FOXP3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Foxp3
• Gene Alias: AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 50943
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ChIP Assay, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q9BZS1
• Gene Symbols: Foxp3
• Immunogen: Synthetic peptide corresponding to residues W(406) T V D E L E F R K K R S Q R(420) of human FOXP3.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov