Fractalkine Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 209401H49

Catalog No. 50-199-1405

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-Fractalkine is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human Fractalkine protein. A BLAST analysis was used to suggest cross-reactivity with Fractalkine from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with Fractalkine from other sources has not been determined.

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

Specifications

• Antigen: Fractalkine
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cx3cl1
• Gene Alias: A-152E5.2; AB030188; ABCD-3; Acc1; AI848747; C3Xkine; chemokine (C-X3-C motif) ligand 1; Cx3c; CX3C membrane-anchored chemokine; C-X3-C motif chemokine 1; C-X3-C motif chemokine ligand 1; Cx3cl1; CXC3; CXC3C; D8Bwg0439e; Fkn; Fractalkine; Neurotactin; NTN; NTT; Processed fractalkine; Scyd1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D, 1; Small-inducible cytokine D1
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P78423
• Gene Symbols: Cx3cl1
• Immunogen: Anti-Fractalkine antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human Fractalkine protein from E. coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 6376
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid