Novus Biologicals™ G-CSF Antibody Pair [HRP]

Description

Monoclonal/Polyclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Human G-CSF.

Specifications

• Assay Range: 93.75 pg/mL
• Assay Sensitivity: 93.75-6000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Human G-CSF.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: C17orf33, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), CSF3OS, filgrastim, G-CSF, GCSFlenograstim, granulocyte colony-stimulating factor, Lenograstim, MGC45931, pluripoietin
• Gene ID (Entrez): 1440
• Gene Symbol: CSF3
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 305 ng of recombinant Human G-CSF.
  Capture Antibody: 1 mg/mL of rabbit anti-Human G-CSF polyclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL of rabbit anti-Human G-CSF monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Biologically Active Proteins, Cell Cycle and Replication, Cytokine Research
• Storage Requirements: Storage is content dependent.
• Target: G-CSF
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 6000 pg/mL is recommended.