Invitrogen™ GAD65/GAD67 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA184572

Catalog No. PA184572

Description

A suggested positive control for immunohistochemical applications is brain. In Western blot, a doublet at ~65/67 kDa has been observed. Rabbit anti Glutamate Decarboxylase 1/2 antibody recognizes both molecular forms of glutamate decarboxylase, GAD1 (GAD67) and GAD2 (GAD65), the enzyme which converts glutamic acid to gamma -aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.

GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA ( gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Specifications

• Antigen: GAD65/GAD67
• Classification: Polyclonal
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P14748, P18088, P48318, P48320, Q05329, Q05683, Q99259
• Gene Symbols: Gad1, GAD2
• Immunogen: Synthetic peptide sequence DFLIEEIERLGQDL from the C-terminus of GAD.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Feline
• Product Type: Antibody
• Isotype: IgG

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Gene: Gad1
• Gene Alias: 6330404F12Rik; 65 kDa glutamic acid decarboxylase; 67 kDa glutamic acid decarboxylase; CPSQ1; EC 4.1.1.15; EP10; FLJ45882; GAD; GAD 1; GAD 2; GAD 65; GAD 67; GAD(65); GAD1; Gad-1; Gad2; Gad-2; GAD25; GAD44; Gad65; GAD-65; GAD67; GAD-67; Glutamate decarboxylase 1; Glutamate decarboxylase 1 (brain); glutamate decarboxylase 1 (brain, 67kDa); glutamate decarboxylase 1 variant GAD67NT; glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase 67 kDa isoform; GLUTAMATE DECARBOXYLASE, 67 KD ISOFORM (GAD-67) (67 KD GLUTAMIC ACID DECARBOXYLASE); Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase; glutamic acid decarboxylase 1; glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; SCP
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 101082244, 14415, 14417, 24379, 24380, 2571, 2572, 493699
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid