Invitrogen™ GAL4 Monoclonal Antibody

Catalog No.	Quantity
33-860-0	200 μg

Catalog No. 33-860-0 Supplier Invitrogen™ Supplier No. 338600

Description

Mouse Monoclonal Antibody

33-8600 detects the Gal4 DNA Binding Domain by western blot, immunoprecipitation and immunofluorescence corresponding to amino acids 1-147 of the GAL4 DNA binding region.

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a 'zinc finger' sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

Specifications

• Antigen: GAL4
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.1% sodium azide; pH 7.4
• Gene: GAL4
• Gene Accession No.: P04386
• Gene Alias: antigen NY-CO-27; GAL4; Gal-4; GAL81; galactose-responsive transcription factor GAL4; galectin 4; galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside binding soluble 4; lectin, galactoside-binding, soluble, 4; LGALS4; Regulatory protein GAL4; YPL248C
• Gene Symbols: GAL4
• Host Species: Mouse
• Immunogen: A recombinant protein corresponding to amino acids 1-147 mapping within the amino terminal, DNA binding domain of GAL4.
• Purification Method: Protein A
• Quantity: 200 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 855828
• Target Species: Yeast
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ