Invitrogen™ GAPDHS Monoclonal Antibody (Hs-8)

Catalog No.	Quantity
01-675-999	100 μg

Catalog No. 01-675-999 Supplier Invitrogen™ Supplier No. MA119235

Description

Mouse Monoclonal Antibody

This antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein. For ICC staining, membrane permeabilization with acetone is essential. Purity is >95% by SDS-PAGE.

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important 'house keeping' functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

Specifications

• Antigen: GAPDHS
• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: Hs-8
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 15mM sodium azide; pH 8
• Gene: GAPDHS
• Gene Accession No.: O14556
• Gene Alias: epididymis secretory protein Li 278; GAPD2; GAPDH S; GAPDH2; gapdh-2; GAPDHS; GAPDS; Gapd-s; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HEL-S-278; HSD35; HSD-35; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
• Gene Symbols: GAPDHS
• Host Species: Mouse
• Immunogen: Freshly ejaculated human sperms.
• Purification Method: Sequential chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100517097, 26330
• Target Species: Human, Pig
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgM