Invitrogen™ GAPDHS Monoclonal Antibody (Hs-8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA119235

Catalog No. 01-675-999

Description

This antibody reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein. For ICC staining, membrane permeabilization with acetone is essential. Purity is >95% by SDS-PAGE.

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

Specifications

• Antigen: GAPDHS
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 15mM sodium azide; pH 8
• Gene Accession No.: O14556
• Gene Symbols: GAPDHS
• Immunogen: Freshly ejaculated human sperms.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Pig
• Product Type: Antibody
• Isotype: IgM

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: Hs-8
• Conjugate: Unconjugated
• Gene: GAPDHS
• Gene Alias: epididymis secretory protein Li 278; GAPD2; GAPDH S; GAPDH2; gapdh-2; GAPDHS; GAPDS; Gapd-s; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; HEL-S-278; HSD35; HSD-35; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase
• Host Species: Mouse
• Purification Method: sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100517097, 26330
• Content And Storage: 4°C, do not freeze
• Form: Liquid