Invitrogen™ GATA-3 Recombinant Rat Monoclonal Antibody (TWAJ), Alexa Fluor™ Plus 555

Catalog No.	Quantity
74-002-3TP555	50 μL
50-274-5780	20 μL

Catalog No. 74-002-3TP555 Supplier Invitrogen™ Supplier No. 740023TP555

Description

Rat Recombinant Monoclonal Antibody

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Applications Tested: This TWAJ antibody has been tested by immunocytochemistry and flow cytometric analysis of MCF-7 cells. This may be used for immunocytochemistry at 2 μg/mL and for flow cytometry at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 5...

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

Specifications

• Antigen: GATA-3
• Applications: Flow Cytometry, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: TWAJ
• Concentration: 1.0 mg/mL
• Conjugate: Alexa Fluor Plus 555
• Formulation: Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8
• Gene: GATA3
• Gene Accession No.: P23771
• Gene Alias: EGK_19445; GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA3; Gata-3; GATA-binding factor 3; GATA-binding protein 3; HDR; HDRS; jal; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3
• Gene Symbols: GATA3
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 50 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2625
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ