Novus Biologicals™ GDF-15 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human GDF-15.

Specifications

• Assay Range: 4.69 pg/mL
• Assay Sensitivity: 4.69-300 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human GDF-15.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: growth differentiation factor 15, growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC-1NSAID-activated gene 1 protein, MIC1Prostate differentiation factor, NAG-1NSAID-regulated gene 1 protein, NSAID (nonsteroidal inflammatory drug)-activated protein 1, PDFGDF-15, PLABNRG-1, Placental bone morphogenetic protein, Placental TGF-beta, PTGFBPTGF-beta
• Gene ID (Entrez): 9518
• Gene Symbol: GDF15
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-Human GDF-15 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of mouse anti-Human GDF-15 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 15 ng of recombinant Human GDF-15.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Hepatic Stem Cell Markers, Stem Cell Markers
• Storage Requirements: Storage is content dependent.
• Target: GDF-15
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.15 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 300 pg/mL is recommended.