GE Healthcare Amersham Cy3, Cy 5 Maleimide mono-Reactive Dye 5-packs
More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions. Amersham Cy3, Cy 5 Maleimide mono-Reactive Dye packs are packaged in premeasured amounts and foil-sealed to ensure consistent labelings.
Manufacturer: GE Healthcare PA23031
Cy3 and Cy5 mono-functional maleimides are particularly suitable for the selective labeling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides, and oligonucleotides. They are also useful in antibody labeling since the dye molecules are introduced away from the antibody binding site, increasing the chance that functionality of the labeled molecule will be unimpaired. The protocol is simple and robust, offering an alternative chemistry when either greater selectivity of labeling is required or amine labeling with Cy3 and Cy5 NHS esters is not desirable or appropriate.
- Provides an alternative for when CyDye NHS esters are unsuitable.
- Dyes are packaged in premeasured amounts and foil-sealed to ensure consistent labelings
|Labeling 1mg Protein|
|2 - 8°C, protected from light. Reconstituted material may be stored for up to 2 weeks at -20°C in aliquots to avoid repeat freeze thaw cycles.|
|For the labelling of biological compounds with Cy3 monofunctional dye|
|Five vials of dye|
|Cy3 Maleimide Mono-Reactive Dye 5-Pack|
|5 vials each sufficient to label 1mg protein|
|Soluble in cold water and hot water|
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