GE Healthcare Glutathione Sepharose 4 Fast Flow Media
Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
Manufacturer: GE Healthcare 17513203
- Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
- Protein binding capacity: »10mg recombinant GST/mL media (flowrate dependent)
- Binding capacity and high flow properties allow scale-up
- Mild elution conditions preserve protein antigenicity and function
- Stable in all commonly used aqueous buffers
- pH stability: 3 to 12
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|Glutathione and 10-carbon linker arm|
|base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm|
|4 to 30°C, 20% Ethanol|
|Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.|
|10 Carbon spacer arm, 4% Agarose, 90μm particle size|
|Highly cross-linked 4% agarose|
|3 to 12|
|Glutathione Sepharose 4 Fast Flow, 500 mL|
|4% Highly Cross-linked Agarose|
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