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GE Healthcare GSTrap™ HP Columns

Use to provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins. GE Healthcare Life Sciences™ GSTrap™ HP Columns are prepacked with Glutathione Sepharose™ High Performance medium.

Manufacturer:  GE Healthcare 17528202

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Catalog No. 45-000-334


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Description

Description

  • One-step purification of GST-tagged proteins and other glutathione S-transferases or glutathione-dependent proteins
  • Glutathione coupled to Sepharose™ High Performance media
  • Column material: polypropylene
  • May be used with syringe, peristaltic pump or chromatography system
  • Purification process preserves antigenicity and function
  • Available as 1mL and 5mL columns
Media
  • Average particle size: 34µm
  • Ligand: Glutathione
  • Matrix: Highly cross-linked 6% agarose
  • Binding capacity: >7mg/mL
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.
5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Specifications

5mL
16mm
< 20 mL/min
Glutathione
5 x 5 mL
34μm
5 bar [0.5 MPa] (70 psi)
4 to 30°C, 20% Ethanol
0.62 in.
GSTrap HP columns are prepacked with Glutathione Sepharose High Performance resin and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins
Polypropylene
3 to 12
GSTrap HP
5 x 5mL
4% Highly Cross-linked Agarose
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