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Cytiva (Formerly GE Healthcare Life Sciences) HisTrap™ excel Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.   Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

$69.75 - $881.00

Specifications

Product Type HisTrap excel
Material Polypropylene
pH Range 2 to 12
Pressure 5 bar [0.5 MPa] (70 psi)
Storage Requirements 4° to 30°C
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Products 3
Catalog Number Mfr. No. Diameter (Metric) Inner Quantity Flow Rate Price Quantity & Availability  
Catalog Number Mfr. No. Diameter (Metric) Inner Quantity Flow Rate Price Quantity & Availability  
45-003-013 Available on GSA/VA Contract for Federal Government customers only.
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Cytiva (Formerly GE Healthcare Life Sciences)
17371205
7mm 5 x 1mL 1 mL/min - 4 mL/min
Each for $272.00
Add to cart
 
45-003-014 Available on GSA/VA Contract for Federal Government customers only.
View Documents
Cytiva (Formerly GE Healthcare Life Sciences)
17371206
16mm 5 x 5mL 5 mL/min - 20 mL/min
Each for $881.00
Add to cart
 
45-000-505 Available on GSA/VA Contract for Federal Government customers only.
View Documents
Cytiva (Formerly GE Healthcare Life Sciences)
29048586
7mm 1mL 1 mL/min - 4 mL/min
Each for $69.75
Add to cart
 
Description

Description

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
Media
  • Average particle size: 90µm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
Column:
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 x 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 x 25mm
  • Flow rate: 5 to 20mL/min.

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Specifications

Specifications

HisTrap excel
2 to 12
4° to 30°C
Polypropylene
5 bar [0.5 MPa] (70 psi)
6% Highly Cross-linked Agarose
SDS
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