GE Healthcare HisTrap™ HP Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Purify histidine-tagged proteins directly from homogenized, unclarified cells. GE Healthcare Life Sciences™ HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Manufacturer: GE Healthcare 17524802

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Catalog No. 45-000-325

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    Specifications

    Bed Volume 5mL
    Diameter (English) Inner 0.6 in.
    Diameter (Metric) Inner 16mm
    Flow Rate 5mL/min.
    Material Polypropylene
    Packaging Quantity 5 x 5mL
    pH Range 3 to 12
    Product Type HisTrap Column
    Pressure 3 bar
    Quantity 5 x 5mL
    Storage Requirements 4° to 30°C
    Support Type 6% Highly Cross-linked Agarose

    • Prepacked with Ni Sepharose™ High Performance medium
    • Negligible nickel ion leakage
    • Pressure Max: 3 bar
    • Column material: polypropylene
    • 1 and 5mL columns
    • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
    • Manual operation with syringe, pump or chromatography system

    Media
    • Matrix: 6% cross-linked agarose
    • Average particle size: 34µm
    • pH stability working range: 3 to 12

    1mL columns

    • Bed Dimensions: 7 x 25mm
    • Flow velocity: 4mL/min.

    5mL columns
    • Bed Dimensions: 16 x 25mm
    • Flow velocity: 5mL/min.

  • Description & Specifications

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