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GE Healthcare Sephadex™ G10 Media

Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules

Manufacturer:  GE Healthcare 17001002

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Catalog No. 45-000-012

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  • Beads behave as rigid spheres obeying Darcy's Law for purposes of determining maximum flowrate and maximum operating pressure
  • Working pH range 2 to 13
  • Particle size range 55 to 165µm
  • Bed volume in distilled water 2-3mL/g
  • Exclusionation limit: 7 x 102 dextrans, 7 x 102 globular proteins



< Darcy´s Law
Sephadex G-10, 500 g
11.81 in.
24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
2 to 13
Peptides and small biomolecules
4 to 30°C
2 to 3mL/g in Distilled Water
Solid Bead
Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W.
55 to 165μm in wet form, 40 to 120μm in dry form
Cross-linked Dextran
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