Invitrogen GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Manufacturer: Invitrogen A21177
DescriptionSimple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. Competent cells included for added convenience and highest cloning efficiencies.
GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).
The GeneArt CRISPR Nuclease Vector system with competent cells offers
- Easy-to-design genome engineering system
- Affordable, ready-to-use cloning vectors
- Enrichment for hard to transfect or difficult to modify cell lines
Store in freezer (-5 to -30°C)
|Genome editing, gene editing, disease modeling, stem cell research, drug discovery, cell based therapies, gene therapy, gene function, gene tagging, pathway engineering, metabolic engineering, protein production|
|Store in freezer (-5 to -30°C)|
|GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells), 10 rxns, Restriction enzyme/MCS Cloning Method, Restriction Enzyme/MCS Cloning Method, Vector System for Expression of the Functional Components|
|U6 Forward: 5A€²- GGACTATCATATGCTTACCG -3A€²|
|GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with Competent Cells)|
|CRISPR CD4 nuclease vector, linearized, 10X oligonucleotide annealing buffer, DNase/RNase-free water, 5X ligation buffer, T4 DNA ligase, U6 forward sequencing primer, control double-stranded oligonucleotide, OneShot™ TOP10 chemically competent cells|
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