Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit 

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Manufacturer:  Invitrogen™ A21175

Catalog No. A21175

Includes: · CRISPR CD4 Nuclease Vector, linearized
· 10X Oligonucleotide Annealing Buffer
· DNase/RNase-free Water
· 5X Ligation Buffer
· T4 DNA Ligase
· U6 Forward Sequencing Primer
· Control Double-stranded Oligonucleotide

Description

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter.

GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).

GeneArt™ CRISPR Nuclease Vector system offers:

· Easy-to-design genome engineering system

· Affordable, ready-to-use cloning vectors

· Enrichment for hard to transfect or difficult to modify cell

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

Specifications

• For Use With (Application): Genome editing, gene editing, disease modeling, stem cell research, drug discovery, cell based therapies, gene therapy, gene function, gene tagging, pathway engineering, metabolic engineering, protein production
• Includes:
  • CRISPR CD4 Nuclease Vector, linearized
  • 10X Oligonucleotide Annealing Buffer
  • DNase/RNase-free Water
  • 5X Ligation Buffer
  • T4 DNA Ligase
  • U6 Forward Sequencing Primer
  • Control Double-stranded Oligonucleotide
• No. of Reactions: 10
• Storage Requirements: Store in freezer (-5 to -30°C)