Invitrogen GeneArt CRISPR Nuclease Vector with OFP Reporter Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Manufacturer: Invitrogen A21174
DescriptionSimple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter. The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21178).
The GeneArt CRISPR Nuclease Vector system offers:
· Easy-to-design genome engineering system
· Affordable, ready-to-use cloning vectors
· Enrichment for hard to transfect or difficult to modify cell lines
For research use only. Not for use in diagnostic procedures.
Store in freezer (-5 to -30°C)
|Genome editing, gene editing, disease modeling, stem cell research, drug discovery, cell based therapies, gene therapy, gene function, gene tagging, pathway engineering, metabolic engineering, protein production|
|Store in freezer (-5 to -30°C)|
|GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit, 10 rxns, Restriction enzyme/MCS Cloning Method, Restriction Enzyme/MCS Cloning Method, Vector System for Expression of the Functional Components Needed for CRISPR/Cas9 Genome Editing|
|U6 Forward: 5A€²- GGACTATCATATGCTTACCG -3A€²|
|GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit|
|CRISPR OFP Nuclease Vector, linearized: 10X oligonucleotide annealing buffer, DNase/RNase-free water, 5X ligation buffer, T4 DNA ligase, U6 forward sequencing primer, control double-stranded oligonucleotide|
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