Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.

Manufacturer:  Invitrogen™ A24372

Catalog No. A24372


Add to cart

Alert:

For research use only. Not for use in diagnostic procedures.


Includes:
  • 1 bottle Cell Lysis Buffer
  • 1 tube Proteinase K
  • 1 tube PCR Supermix
  • 1 tube Water
  • 1 tube Detection Enzyme
  • 1 tube Detection Reaction Buffer
  • 1 tube Control Template & Primers

Description

Description

The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation. When using genome editing tools to obtain targeted mutations, it is necessary to determine the efficiency at which these nucleases cleave the target sequence, particularly prior to proceeding to the more laborious and expensive processes of cloning and sequencing.

The GeneArt genomic cleavage detection kit contains all of the reagents required prior to gel analysis. A sample of the edited cell population is used as a direct PCR template for amplification with primers specific to the targeted region. The PCR product is then denatured and re-annealed to produce heteroduplex mismatches where double strand breaks have occurred, resulting in indel introduction. These mismatches are recognized and cleaved by the detection enzyme. This cleavage is both easily detectable and quantifiable using gel analysis.

  • Easy—no genomic DNA isolation, direct PCR amplification
  • Rapid—4 hour total processing time
  • Quantative—gel band density directly correlated to target indel introduction

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Recommended Storage

Store all at -5 to -30°C

Specifications

Specifications

Contains:

• 1 bottle Cell Lysis Buffer
• 1 tube Proteinase K
• 1 tube PCR Supermix
• 1 tube Water
• 1 tube T7E1 Detection Enzyme
• 1 tube T7E1 Detection Reaction Buffer
• 1 tube Control Template & Primers

Store all at -5 to -30°C.
Taq Polymerase
INDEL
CRISPR-Cas9, TAL Effector Nuclease
SDS
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit