Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ Precision gRNA Synthesis Kit Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

Manufacturer:  Invitrogen™ A29377

Catalog No. A29377


Add to cart

Alert:

For research use only. Not for use in diagnostic procedures.


Includes:
BOX 1
  • 5X TranscriptAidTM Reaction Buffer 
  • DNase I, RNase-free (1 U/µL) 
  • Nuclease-free water 
  • NTP mix (100 mM each of ATP, GTP, CTP, 
  • UTP in Tris buffer) 
  • Control gRNA forward and reverse primers (10 µM mix) 2 
 
BOX 2
  • Binding Buffer 
  • Wash Buffer 1 (concentrated)3 
  • Wash Buffer 2 (concentrated)4 
  • Nuclease-free water 
  • Gene JETTM RNA Purification Micro Column & Collection Tubes 
  • Elution Tubes, 1.5 mL 

Description

Description

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short ‘one-pot’ PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.

Features of the GeneArt Precision gRNA Synthesis Kit include:
  • Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
  • High yield (>10 ug) and concentration (>200 ng/uL) of gRNA
For research use only. Not for use in diagnostic procedures.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Certifications

CoA

Recommended Storage

gRNA Prep Kit, store at -5 to -30°C and gRNA Cleanup Kit, store at room temperature

Specifications

Specifications

• gRNA Prep Kit, store at -5 to -30°C
• gRNA Cleanup Kit, store at room temperature
T7
Oligos (DNA)
Fast
RNA
Approved for shipment at Room Temperature or on Wet or Dry Ice
>10 µg at >200 ng/µL of gRNA
25 Reactions
SDS
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit