Invitrogen Precision gRNA Synthesis Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.
Manufacturer: Invitrogen A29377
DescriptionSimple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short ‘one-pot’ PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.
- Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
- High yield (>10 ug) and concentration (>200 ng/uL) of gRNA
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gRNA Prep Kit, store at -5 to -30°C and gRNA Cleanup Kit, store at room temperature
|• gRNA Prep Kit, store at -5 to -30°C
• gRNA Cleanup Kit, store at room temperature
|Approved for shipment at Room Temperature or on Wet or Dry Ice|
|>10 µg at >200 ng/µL of gRNA|
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