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Thermo Scientific™ GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA Assay Kit

Catalog No. K1544
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Catalog No. K1544 Supplier Thermo Scientific™ Supplier No. K1544

Description

The GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells contain the human Complement Component 5a Receptor 1 (C5AR1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.

The GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells contain the human Complement Component 5a Receptor 1 (C5AR1) (Accession No. NM_000710) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 (Cat. No. K1213) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element and a promiscuous G Protein, Gα15, stably integrated into CHO-K1 cells.

The C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells cells are functionally validated for Z'-factor and EC50 concentrations using C5a. In addition, C5AR1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_001736.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ C5AR1-Gα15 CHO-K1DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ C5AR1-Gα15 CHO-K1 Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
Gene Symbol C5aR1
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 1 Plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry C5AR1
Type Division-Arrested Cells Assay Kit
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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