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Thermo Scientific™ GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 Cells

Catalog No. K1746
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Catalog No. K1746 Supplier Thermo Scientific™ Supplier No. K1746
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Description

GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells contain the human Complement Component 5a Receptor 1 (C5aR1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.

GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells contain the human Complement Component 5a Receptor 1 (C5aR1) (Accession No. NM_000710) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 (Cat. No. K1537) contains a beta-lactamase reporter gene under control of a NFAT response element and a promiscuous G Protein, Gα15, stably integrated into CHO-K1 cells.

The GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of C5a. In addition, GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_001736.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells
• GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ C5aR1-Gα15-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol C5aR1
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry C5AR1
Type CHO-K1 Cells
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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