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Thermo Scientific™ GeneBLAzer™ CCKAR HEK 293T DA Assay Kit

Catalog No. K1623
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8 x 106 Cells
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K1623 8 x 106 Cells
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Catalog No. K1623 Supplier Thermo Scientific™ Supplier No. K1623

Description

The GeneBLAzer™ CCKAR-NFAT-bla CHO-K1 cells contain the human Cholecystokinin A Receptor (CCKAR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line.

The GeneBLAzer™ CCKAR-NFAT-bla CHO-K1 cells contain the human Cholecystokinin A Receptor (CCKAR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line. GeneBLAzer™ NFAT-bla CHO-K1 (Cat. No. K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.

The CCKAR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, CCKAR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000730.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ CCKAR HEK 293T DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ CCKAR HEK 293T DA Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ CCKAR HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
Gene Symbol CCKAR
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 8 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry CCKAR
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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