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Thermo Scientific™ GeneBLAzer™ MC2R CHO-K1 DA Assay Kit

Catalog No. K1597
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Catalog No. K1597 Supplier Thermo Scientific™ Supplier No. K1597

Description

The GeneBLAzer™ MC2R CHO-K1 DA (Division Arrested) cells contain the human melanocortin-2 receptor (MC2R), and the Melanocortin-2 receptor accessory protein (MRAP), stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line.

The GeneBLAzer™ MC2R CHO-K1 DA (Division Arrested) cells contain the human melanocortin-2 receptor (MC2R), (Accession No. NM_000529.1) and the Melanocortin-2 receptor accessory protein (MRAP), (Accession No. NM_178817.3) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. No. K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP Response Element (CRE).

The MC2R-CHOK1 DA cells are functionally validated for Z'-factor and EC50 concentrations of ACTH 1-24. In addition, MC2R-CRE-bla-CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000529.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ MC2R CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ MC2R CHO-K1 Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ MC2R CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
Gene Symbol MC2R
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 1 Plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry MC2R
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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