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Invitrogen™ GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit

Catalog No. K1619
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8 x 106 cells
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Catalog No. Quantity
K1619 8 x 106 cells
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Catalog No. K1619 Supplier Invitrogen™ Supplier No. K1619

Description

GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit

The GeneBLAzer™ PAC1-CRE-bla CHO-K1 cells contain the human Adenylate Cyclase Activating Polypeptide 1 Receptor 1 (ADCYAP1R⁄PAC1) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat #K1129) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE). The PAC1-CRE-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of established ligands. In addition, PAC1-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_001118
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage K1619 GeneBLAzer™ PAC1 CHO-K1 DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ PAC1 CHO-K1 DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70μg
• Solution D
• GeneBLAzer™ PAC1 CHO-K1 DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ PAC1 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
Gene Symbol ADCYAP1R1
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 8 x 106 cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry ADCYAP1R1, PAC1
Type Division-Arrested Cells Assay Kit
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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