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Thermo Scientific™ GeneBLAzer™ PPAR gamma DA Assay Kit

Catalog No. K1419
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K1419 1 plate
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Catalog No. K1419 Supplier Thermo Scientific™ Supplier No. K1419

Description

GeneBLAzer™PPAR gamma DA (Division Arrested) cells and PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line.

GeneBLAzer™PPAR gamma DA (Division Arrested) cells and PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line. GeneBLAzer™UAS-bla HEK 293H cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both PPAR gamma DA cells and PPAR gamma-UAS-bla HEK 293H cells are functionally validated for Z' and EC50 concentrations of Rosiglitazone. In addition, PPAR gamma-UAS-bla HEK 293H cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
• PPAR gamma 293H DA cells (K1419A)
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Gene ID (Entrez) 5468
Gene Symbol PPARγ
Primary Agonist Calcitriol
Product Line GeneBLAzer
Quantity 1 plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry PPARG, PPAR gamma, NR1C3
Type Division-Arrested Cells Assay Kit
Cell Line 293H
System Type GeneBLAzer™
Druggable Target Nuclear Receptors
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For Research Use Only. Not for use in diagnostic procedures.

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