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Invitrogen™ GFP Monoclonal Antibody (3E6)
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Catalog No. A11120
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100 μg
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A11120 100 μg
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Catalog No. A11120 Supplier Invitrogen™ Supplier No. A11120
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Description

Mouse Monoclonal Antibody

Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibodies in 0.5mL PBS, pH 7.4. Store solution for up to 3months at 4°C with the addition of 2mM sodium azide.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
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Specifications

Antigen GFP
Applications ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 3E6
Conjugate Unconjugated
Formulation PBS with BSA and no preservative
Gene Alias GFP; GFP tag; GFP2; green fluorescence; green fluorescent; green fluorescent protein; Turbo eGFP
Host Species Mouse
Immunogen The GFP was isolated directly from the jellyfish Aequorea victoria.
Purification Method Protein G
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
Isotype IgG2a
Target Molecule Tag
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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