Invitrogen™ GFP Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5109258

Catalog No. PIPA5109258

Description

Positive Control: GFP11-tagged fusion protein.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
• Immunogen: Synthetic peptide immune sequence is RDHMVLHEYVNAAGITC.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Tag

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.2% BSA, 50% glycerol and 0.05% sodium azide; pH 7.4
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov