Gibco™ Cell Dissociation Buffer, enzyme-free, PBS

Catalog No.	Quantity
13-151-014	100 mL

Catalog No. 13-151-014 Supplier Gibco™ Supplier No. 13151014

Description

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

Gibco Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). It is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent cells.

Gibco Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS). Gibco Cell Dissociation Buffer is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Gibco Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent cells.

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

• Chelators: EDTA
• Content And Storage: Storage conditions: 15°C to 30°C
  Shipping conditions: Room temperature
  Shelf life: 24 months from date of manufacture
• Cell Line: HeLa, NIH 3T3
• Classification: Animal Origin-free
• Product Type: Cell Culture Dissociation Reagent
• Sterility: Sterile-filtered
• With Additives: EDTA
• Quantity: 100 mL
• Remarks: Room Temperature Stable
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

What is the osmolality and pH range for Cell Dissociation Buffer, enzyme-free, PBS (Cat. No. 13151014)

Below are QC test specifications for pH and osmolality for this product:

- pH: 6.9 to 7.9
- Osmolality: 289 to 321 mOsm/kg

At what confluency should cells be at prior to their dissociation using the Cell Association Buffer, enzyme-free, PBS?

The buffer should be used with cells that are sub-confluent (60-80%). If cells are more than 80% confluent, it will be hard to detach them.

Will the Cell Dissociation Buffer, enzyme-free, PBS damage cell surface markers/receptors?

This buffer is very gentle. It should preserve the structural and functional integrity of cell surface proteins. However, we recommend that the customer test their cells and proteins.

Can I use the Cell Dissociation Buffer, enzyme-free, PBS to dissociate cells?

This Enzyme-Free Cell Dissociation Buffer does not rely on proteolysis for its action. Therefore, it is not suitable for strongly adherent cells, or for routine passage of some cell types (e.g., A431, Hep2, A549, MDCK, WI-38). This product is intended to gently dissociate cells from attachment substrates and each other while maintaining surface protein integrity.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.