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Dissociation Reagents

Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

Corning™ Accutase™ Cell Detachment Solution
SDP

For detachment of adherent cells, primary stem cell lines and tissues

2

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium and Magnesium
SDP

Used to enzymatically release adherent cells from tissue culture plates for passaging.

3

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate
SDP

Used to enzymatically release adherent cells from tissue culture plates for passaging.

4

Cytiva HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

Content And Storage Store frozen
Sterilization Method 0.1 μm Sterile-filtered
With Additives Trypsin, porcine,Hanks’ Balanced Salt solution (HBSS),EDTA
Form Liquid
Without Additives No Calcium,No Magnesium
Shelf Life 24 Months
Source Porcine pancreas
5

Corning™ EDTA
SDP

6

BD Cell Detachment Solution

Replacement for trypsin solution and can be used for the passaging of cell lines

7

Gibco™ Trypsin-EDTA (0.25%), phenol red
GREENER_CHOICE

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

ENCOMPASS_PREFERRED
Chelators EDTA
Content And Storage Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Form Liquid
Tested For In vitro Bioassay
Green Features Sustainable packaging
Osmolality 270 - 320 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco™
8

Corning™ 2.5% Trypsin in HBSS w/o Calcium, Magnesium and Phenol Red
SDP

Enzymatic dissociation reagents tested for porcine parvovirus

9

Cytiva HyrTrp Cell Dissociation Reagent

Recombinant and animal-derived component-free alternative to traditional porcine trypsin

Content And Storage -20 °C
For Use With Attachment-dependent cell types MRC-5, Vero, CHO K-1 cell lines grown with or without serum
Product Type Cell Culture Dissociation Reagent
Shelf Life 12 months from manufacturing date when stored at -20°C
10

MilliporeSigma™ Trypsin 0.25% and 1mmol EDTA

The Trypsin-EDTA, In Hank's Balanced Salt Solution, 0.25% Trypsin and 1mM EDTA, without Ca 2+ and Mg 2+ is available in a 100mL format.

11

Corning™ CellStripper Dissociation Reagent
SDP

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

12

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red

Unlike porcine trypsin, free of animal-derived components

ENCOMPASS_PREFERRED
Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Green Features Energy efficient, Sustainable packaging
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco™
13

MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x)

Used to dissociate primary cells, tissues, and tumors with an increased plating efficiency. MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x) is PBS based formulation of chelating agents that contain agents to stabilize their activity on cells.

Content And Storage Store at room temperature or at 4°C.
Product Type Cell Culture Dissociation Reagent
Form Liquid
Formulation Chelating and stabilizing agents
Shelf Life 2 Years
Concentration 1 X
For Use With (Application) Cell Dissociation
Sterility Sterile
14

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium
SDP

Enzymatic dissociation reagents tested for porcine parvovirus