Gibco™ Dispase II, powder

Catalog No.	Quantity
17-105-041	5 g

Catalog No. 17-105-041 Supplier Gibco™ Supplier No. 17105041

Description

Amino-endo peptidase that hydrolyzes N-terminal peptide bonds of non-polar amino acid residues

Dispase II has a mild proteolytic action that makes it useful for the isolation and routine passaging of primary cells. Dispase II is relatively gentle, dissociates well at physiological temperature and pH, and generally maintains cell membrane integrity.

• Produced in Bacillus polymyxa
• Packaged as lyophilized, non-sterile powder for research use in cell or tissue dissociation, and to prevent clumping in suspension cultures
• Activity is >0.5 units/mg

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2°C to 8°C. Protect from light.
  Shipping conditions: Room temperature
  Shelf life: 24 months from date of manufacture
• Form: Lyophilized
• Product Type: Cell Culture Dissociation Reagent
• Sterility: Non-sterile
• Quantity: 5 g
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

What is the stability of reconstituted Dispase?

The Dispase solution is stable at -5 to -20 degrees C for a minimum of 1 month.

I have Dispase, Cat. No. 17105041. Is it Dispase I or II?

This product is Dispase II.

Why is collagenase type IV favored over dispase even though the dissociation with collagenase IV seems to take longer (between 30 and 60 min, depending from the lot, at 37 degrees C) compared to dispase?

Actually, in a feeder-based culture, dispase (2 mg/mL) should take about 15-25 min to work at 37 degrees C. Two to three minutes' dissociation time would apply to feeder-free cultures. Dispase is a more aggressive enzyme, so it works faster, but that also means that when the PSC clumps are harvested, they are more sensitive to being broken apart by trituration. Once the clumps are harvested, they should be pipetted up and down a few times to break up the clumps to the appropriate size. If the cells are harvested with collagenase type IV, they have to be pipetted more times because the clumps are harder to break up, but this means that there is less likelihood to break up the clumps into pieces that are too small. If the cells are harvested with dispase, they have to be pipetted fewer times, and care has to be taken to ensure that the clumps are not broken too much. Either enzyme is fine to use, and if you have enough experience, you may prefer to use dispase to save time. But for a less experienced user, we recommend using collagenase type IV as it is safer and you are less likely to ruin your culture by over-triturating.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.