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Gibco™ FreeStyle™ CHO Expression Medium Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Animal origin-free, chemically defined, protein-free medium

Manufacturer:  Gibco™ 12651014

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Catalog No. 12-651-014


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Description

Description

FreeStyle CHO Expression Medium is specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.

  • Formulation without hypoxanthine and thymidine for use with or without dhfr systems
  • Ability to support high-density suspension growth and transient transfection of CHO-S cells
  • Animal origin-free, protein-free and chemically defined formulation
  • Easy use as part of the FreeStyle MAX CHO Expression System for protein production
  • Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems

  • Gibco FreeStyle CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems
  • Non-dhfr systems require the addition of HT Supplement before use
Ability to support high-density suspension growth and transient transfection of CHO-S cells
  • Gibco FreeStyle CHO cells in suspension can be cultured directly into Gibco FreeStyle CHO Expression Medium without adaptation
  • Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle CHO Expression Medium, usually without any further adaptation
  • Cells usually require adaptation from serum-containing formulations
  • Cells cultured in Gibco FreeStyle CHO Expression Medium can be transfected using lipid-based transfection reagents, such as FreeStyle MAX reagent
Animal origin-free, protein-free and chemically defined formulation
  • Gibco FreeStyle CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
  • Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition
Scalability in spinner flasks and bioreactors
  • Protein production using Gibco FreeStyle CHO Expression Medium can be scaled up in spinner flasks or bioreactors
  • The appropriate spinner or impeller speed and seeding density should be optimized for each system
  • Depending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

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Specifications

Specifications

Chemically Defined, Protein-Free, Serum-Free
Suspension Cell Culture
Liquid
No Phenol Red
Serum-Free
FreeStyle™ CHO-S Cells, CHO
1000mL
Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
≤0.25 EU/mL, ≤ 0.25 EU/ml
No Glutamine
Freestyle™, Gibco®
Room Temperature
Bottle(s)
Documents
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For Research Use Only. Not for use in diagnostic procedures.