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Gibco™ Amphotericin B
Description
- Prevents growth of fungi by causing an increase in fungal plasma membrane permeability
- Gibco™ Fungizone™ Antimycotic is used to prevent contamination of cell cultures by yeast and multicellular fungi
- Contains 250μg of amphotericin B and 205μg of sodium deoxycholate per mL of distilled water
- Recommended working concentration ranges from 0.25 to 2.50μg/mL
Specifications
Specifications
| Content And Storage | Storage conditions: -5°C to -20°C Shipping conditions: Frozen on dry ice Shelf life: 12 months from date of manufacture |
| Concentration | 0.25 to 2.5 μg/mL |
| Form | Liquid |
| Product Type | Antifungal |
| Sterility | Sterile-filtered |
| For Use With (Application) | Prevention of Cell Culture Contamination |
| Product Line | Fungizone |
| Quantity | 20 mL |
| Shipping Condition | Dry Ice |
Frequently Asked Questions (FAQs)
Amphotericin B can be freeze-thawed 2-3 times without appreciable loss of potency.
Amphotericin B in solution is stable at 2-8 degrees C for approximately 4 weeks.
Amphotericin B is the generic version of Fungizone. Fungizone is a trademark of E.R. Squibb & Sons, LLC.
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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