Gibco™ MEM α, no nucleosides

Minimum Essential Medium is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells

Supplier:  Gibco™ 12561072

Catalog No. 12-561-072

Description

MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.

• Modified as follows: with phenol red, L-glutamine; without ribonucleosides, deoxyribonucleosides
• Modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B₁₂, biotin, and ascorbic acid
• Available without nucleosides for use as selection medium for DG44 and other DHFR-negative cells
• Made with Earle's salts
• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
• Uses sodium bicarbonate buffer system (2.2g/L), and therefore requires 5-10% CO₂ environment to maintain physiological pH
• Sustainable packaging

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: MEM α (Minimum Essential Medium α)
• Sterility: Sterile-filtered
• Without Additives: No HEPES, No Ribonucleosides, No Deoxyribonucleosides
• Product Line: Gibco™
• Shipping Condition: Room Temperature

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Classification: Animal Origin-free
• Form: Liquid
• Serum Level: Standard Serum Supplementation
• With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
• Green Features: Sustainable packaging
• Quantity: 10 x 500 mL

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture