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Gibco™ MEM α, nucleosides
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Catalog No. 12571089
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Description

Requires

Supplementation

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells.

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With: Ribonucleosides, Deoxyribonucleosides, Phenol Red, L-glutamine

The complete formulation is available.

Using MEM α

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle's salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

TRUSTED_SUSTAINABILITY

Specifications

Cell Line HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type Primary Rat Astrocytes
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type MEM α (Minimum Essential Medium α)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides
Without Additives No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 6 x 1000 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

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