Gibco RPMI 1640 Medium, HEPES
Originally developed to culture human leukemic cells in suspension and as monolayer
Manufacturer: Gibco 22400097
Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. This RPMI is modified as follows: with L-glutamine, HEPES, phenol red.
- Contains reducing agent glutathione, high concentrations of vitamin biotin, vitamin B12, and PABA
- Vitamins inositol and choline are present in high concentrations
- Contains no proteins, lipids, or growth factors
- Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
- Sustainable packaging
Cell Culture, Mammalian Cell Culture
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|Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
|Standard Serum Supplementation|
|No Sodium Pyruvate|
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Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For in vitro diagnostic use.