Gibco™ Trypsin-EDTA (0.25%), phenol red

Catalog No.	Quantity
25-200-056	100 mL
25-200-072	500 mL
25-200-114	20 x 100 mL

Catalog No. 25-200-056 Supplier Gibco™ Supplier No. 25200056

Description

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements.

• Solutions tested for pH, osmolality, sterility, and performance
• Raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis
• Documented traceability available including lot traceability, animal origin certificates, lot analyses, irradiation certificates, viral inactivation summary, and supply chain transparency

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Dry Ice

Compliance

Manufactured at a cGMP compliant facility registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards.

Specifications

• Chelators: EDTA
• Content And Storage: Storage conditions: -5°C to -20°C
  Shipping conditions: Wet ice
  Shelf life: 24 months from date of manufacture
• Cell Type: Mammalian Cells
• Classification: Animal Origin
• Concentration: 1X
• Form: Liquid
• Product Type: Cell Culture Dissociation Reagent
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: Phenol Red, EDTA
• Osmolality: 270 - 320 mOsm/kg
• pH: 7.2 to 8
• Product Line: Gibco
• Quantity: 100 mL
• Shipping Condition: Wet Ice
• Source: Animal Origin
• Tested For: In vitro Bioassay

Frequently Asked Questions (FAQs)

What are the storage conditions for the Trypsin-EDTA (0.25%), phenol red (Cat. No. 25200072)?

We recommend storing the Trypsin-EDTA (0.25%), phenol red (Cat. No. 25200072) at  -5°C to -20°C as specified in the Contents & Storage section of the Thermofisher Scientific product page for this product. Although it is possible to store the trypsin for up to one week at +4°C, we would recommend preparing aliquots and storing them at -5°C to -20°C. These aliquots should be used up after thawing and freeze/thaw cycles should be avoided.

How long can I keep trypsin in the refrigerator after thawing?

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.

What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.