Invitrogen™ Glucocorticoid Receptor Polyclonal Antibody

Catalog No.	Quantity
PA1510A	200 μL

Catalog No. PA1510A Supplier Invitrogen™ Supplier No. PA1510A

Description

Rabbit Polyclonal Antibody

PA1-510A detects glucocorticoid receptor (GR) from human, rat, and reptile tissues. This product detects both the unactivated and activated forms of the receptor. PA1-510A has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR from rat liver extract. PA1-510A immunizing peptide corresponds to amino acid residues 150- 175 from human GR.

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity.

Specifications

• Antigen: Glucocorticoid Receptor
• Applications: Gel Shift, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: Whole serum, PBS with 0.05% sodium azide
• Gene: Nr3c1
• Gene Accession No.: P04150, P06536, P06537
• Gene Alias: cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038
• Gene Symbols: Nr3c1
• Host Species: Rabbit
• Immunogen: Synthetic Peptide: A(150) P T E K E F P K T H S D V S S E Q Q H L K G Q T G(175).
• Quantity: 200 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 14815, 24413, 2908
• Target Species: Human, Mouse, Rat, Reptile
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG