Glutamate Dehydrogenase Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 20041580100

Catalog No. 50-199-1031

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-GLUTAMATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver]. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Glutamate dehydrogenase, GluD1, is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

Specifications

• Antigen: Glutamate Dehydrogenase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GLUD1
• Gene Alias: Ac2-281; AI118167; BOS_24262; epididymis tissue sperm binding protein Li 18mP; GDH; GDH 1; Gdh1; Gdh-X; Glud; Glud1; Gludeha; Gludl; glutamate dehydrogenase; glutamate dehydrogenase (NAD(P)+); glutamate dehydrogenase 1; Glutamate dehydrogenase 1, mitochondrial; glutamate dehydrogenase 1-like; LOC100351029; Memory-related gene 2 protein; MRG-2
• Host Species: Rabbit
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Bovine
• Product Type: Antibody

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P00366
• Gene Symbols: GLUD1
• Immunogen: Glutamate Dehydrogenase [Bovine Liver]
• Regulatory Status: RUO
• Gene ID (Entrez): 281785
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized