Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Catalog No.	Quantity
45-000-287	500 mL
45-000-286	100 mL
45-000-285	25 mL

Catalog No. 45-000-287 Supplier Cytiva Supplier No. 17513203

Description

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

• Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
• Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
• Binding capacity and high flow properties allow scale-up
• Mild elution conditions preserve protein antigenicity and function
• Stable in all commonly used aqueous buffers
• pH stability: 3 to 12

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Binding Capacity: >10 mg/mL
• Description: Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
• Flow Rate: <450 cm/hr.
• Kit Contents: 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
• Matrix: Highly cross-linked 4% agarose
• pH Range: 3 to 12
• Support Type: 4% Highly Cross-linked Agarose
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Max. Operating Pressure: base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Particle Size: 90 μm
• Quantity: 500 mL
• Type: Chromatography Media
• Ligand Type: Glutathione and 10-carbon linker arm