Invitrogen™ GM-CSF Monoclonal Antibody (1089), Biotin

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ M501B

Catalog No. ENM501B

Description

M501B targets Human GM-CSF in ELISA applications and shows reactivity with Human samples. The M501B immunogen is glycosylated, CHO-derived recombinant human GM-CSF. M501B detects Human GM-CSF which has a predicted molecular weight of approximately 14 kDa. The M501B GMCSF antibody (biotinylated conjugate of clone 1089) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M500A (clone 3092). Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Biotinylated antibody M501B (clone 1089) and antibody M500A (clone 3092) have successfully been used in combination with recombinant GMCSF protein SGMCSF in ELISA applications.

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

Specifications

• Antigen: GM-CSF
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 4% BSA and Proprietary Preservative
• Gene Accession No.: P04141
• Gene Symbols: CSF2
• Immunogen: Glycosylated, CHO-derived recombinant human GM-CSF.
• Quantity: 250 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: ELISA
• Clone: 1089
• Conjugate: Biotin
• Gene: CSF2
• Gene Alias: colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; molgramostin; put. GM-CSF; sargramostim
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 1437
• Content And Storage: -20°C
• Form: Liquid