Invitrogen™ Goat anti-Human IgG (Heavy chain) Secondary Antibody

Goat Polyclonal Secondary Antibody

Supplier:  Invitrogen™ 31118

Catalog No. PI31118

Description

Product # 31118 has been successfully used in Western blot, IF, ICC, IHC, ELISA, and ISH applications. Product # 31118 reacts with the heavy chains of human IgG, but does not react against non-immunoglobulin serum proteins. Based on solid-phase enzyme immunoassay, this antibody has minimal cross-reactivity with human IgE and human IgM. However, this antibody may cross-react with immunoglobulins from other species. The activity measured by a solid phase binding assay is 91%.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (Heavy chain)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Host Species: Goat
• Quantity: 500 μg
• Primary or Secondary: Secondary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
• Form: Liquid

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, In Situ Hybridization (ISH), ELISA
• Concentration: 1 mg/mL
• Formulation: PBS with 20mg/mL sucrose and 0.08% sodium azide; pH 7.8
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Secondary Antibody
• Isotype: IgG

Safety and Handling

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