Invitrogen™ Goat anti-Human IgG (Kappa light chain) Recombinant Secondary Antibody

Goat Recombinant Monoclonal Secondary Antibody

Supplier:  Invitrogen™ A56854

Catalog No. A56854

Description

This Goat-anti-Human IgG (Kappa Light Chain), Recombinant Secondary Antibody specifically detects human IgG Kappa Light chain with no cross-reactivity to Lambda light chain, heavy chain, and other species immunoglobulins. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity, animal origin-free formulation, and lot-to-lot consistency. The sensitivity and specificity of each lot are confirmed using ELISA. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (Kappa light chain)
• Classification: Recombinant Monoclonal
• Concentration: 1.0 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Immunogen: Purified Human IgG.
• Quantity: 250 μL
• Primary or Secondary: Secondary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ELISA, Immunocytochemistry, Western Blot
• Clone: 81H59L2
• Conjugate: Unconjugated
• Host Species: Goat
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Human
• Product Type: Secondary Antibody
• Isotype: IgG

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov