Invitrogen™ Goat anti-Human IgG (Lambda Light Chain) Recombinant Secondary Antibody, Alexa Fluor™ Plus 488

Goat Recombinant Monoclonal Secondary Antibody

Catalog Number	Mfr. No.	Quantity
A56867	Invitrogen™ A56867	250 μL
A5686750UL	Invitrogen™ A5686750UL	50 μL

Description

This Goat-anti-Human IgG (Lambda Light Chain), Recombinant Secondary Antibody specifically detects human IgG Lambda Light chain with no cross-reactivity to kappa light chain, heavy chain, and other species immunoglobulins. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency. The sensitivity and specificity of each lot is confirmed using ELISA. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Human IgG (Lambda Light Chain)
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Conjugate: Alexa Fluor Plus 488
• Host Species: Goat
• Regulatory Status: RUO
• Formulation: proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.5
• Primary or Secondary: Secondary
• Purification Method: Affinity chromatography

• Clone: 85H45L7
• Content And Storage: 4°C, store in dark
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Immunogen: Purified Human IgG.
• Product Type: Secondary Antibody