Goat anti-Rat IgG Fc Secondary Antibody, DyLight 649 (Pre-adsorbed), Novus Biologicals™

Goat Polyclonal Antibody

Supplier:  Novus Biologicals NBP151886

Catalog No. NBP151886

Description

IgG Fc Polyclonal antibody specifically detects IgG Fc in Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin).

Specifications

• Antigen: IgG Fc
• Classification: Polyclonal
• Conjugate: DyLight 649
• Host Species: Goat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Test Specificity: Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species.
• Content And Storage: Store at 4C. Do not freeze.

• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Concentration: 0.5 mg/mL
• Dilution: Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:50 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:500
• Immunogen: Rat IgG-Fc Fragment
• Quantity: 1 mL
• Primary or Secondary: Secondary
• Target Species: Rat
• Isotype: IgG

For Research Use Only